Blue pippin. Geographic variation in West African Agama picticauda: insig...

A PacBio Sequencing library was constructed using the SMRTbell T

Based on the results, we combined pools at equimolar concentrations and size selected for a range of 250-600 bp with a Blue Pippin device (Sage Science, Inc.). In the other laboratory (laboratory 2), we measured DNA quantity with Qubit, examined fragment distributions with TapeStation, and then size-selected pools showing excessive large ...Pippin is an electronica music producer based in New Zealand. His genre-bending sound can't be categorised by just one box, as it is a unique blend of 2-step, dubstep, trip-hop and indie …Size select the DNA with Blue Pippin, using High Pass Plus gel cassette and 0.75% Dye Free for 1-10 kb External Standards S1 according to manufacturer's protocol. Further, 10 μg of DNA is required for each lane, and typically the amplified sample is enough for two lanes. Adjust the cutoff length depending on the fragment size distribution.Sage Science size selecting with bluepippin Size Selecting With Bluepippin, supplied by Sage Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.Double digest restriction-site associated sequencing (ddRAD-seq) is a flexible and cost-effective strategy for providing in-depth insights into the genetic architecture of germplasm collections.The Blue Pippin applies pulsed-field electrophoresis technology to size select DNA for downstream applications (i.e. library preparation). This machine is extremely beneficial for high molecular weight applications due to its high pass filter that allows you to collect all fragments above a specified threshold.Conductivity / pH Meter. GE Biacore 3000 Surface Plasmon Resonance (SPR) Malvern NanoSight NS300. Malvern Zetasizer Nano ZS. Milli-Q Advantage A10 Water System. MiSonix Ultra Sonicator. New Brunswick Innova 44R Shakers. Other Analytical Core Instruments. Shimadzu UV-1800 UV-Vis Spectrophotometer.The nGBS libraries were then size selected on Blue Pippin, discarding fragments smaller than 300 bp and larger than 500 bp long. Sequencing was carried out on an Illumina NextSeq 500/550 using V2 Chemistry and 300 cycles. Sequences were demultiplexed with Illumina’s CASAVA data analysis software and proprietary software …This instructional video from Sage Science guides you through the process of preparing your cassette for use with either the Pippin Prep or BluePippin automa...Pippin definition, any of numerous roundish or oblate varieties of apple. See more.The Blue Pippin facilitates library construction for most popular NGS platforms, and is recommended by Pacific Biosciences, Illumina and Ion Torrent for certain workflows. Utilizing pulsed-field electrophoresis it is an ideal solution for resolving and collecting high molecular weight DNA up to 50kb. The platform features the ability to collect ...Note, however, that the CHEF Mapper and Pippin Pulse systems require at least 50 ng of DNA for analysis and are recommended only if there is sufficient DNA sample available. DNA Sizing QC Method Comments Procedure . Femto Pulse Highly recommended (Requires 200-500 picograms) Agilent Technologies, Inc.If you'd like us to Pippin your samples for you (plus perform quantification and fragment analysis), the cost is $75 per sample. CLICK HERE to schedule a reservation on BluePippin! (Note, you will need to register for an iLab account.) Fragment Analyzer traces of 5 different Blue Pippin size selected Illumina libraries.Pippin is a 1972 musical with music and lyrics by Stephen Schwartz and book by Roger O. Hirson. Bob Fosse, who directed the original Broadway production, also contributed to the libretto.The musical uses the premise of a mysterious performance troupe, led by the Leading Player, to tell the story of Pippin, a young prince on his search for meaning and significance.Sling Blue TV is an amazing streaming service that offers a wide variety of channels for your viewing pleasure. With Sling Blue TV, you can access over 50 popular channels, including sports, news, entertainment, and more.Page 6 P/N 100-286-000-07 STEP Purify DNA Notes 1 Add 0.45X volume of AMPure PB beads to the End-Repair reaction. (For detailed instructions on AMPure PB bead purification, see the Concentrate DNA section.) 2 Mix the bead/DNA solution thoroughly. 3 Quickly spin down the tube (for 1 second) to collect the beads. Do not pellet beads.The Sage Science Blue Pippin is a preparative electrophoresis platform that automatically separates and extracts DNA fragments based on preselected fragment size or size range. Manual gel extraction is not necessary. Using pre-cast disposable gel cassettes with internal size markers, DNA is automatically collected in buffer according to the ...The larger sample wells now allow a maximum load of 10 μg (100% up from standard Blue Pippin cassettes). Also the size of the elution module has been increased as well as the surface area of the filtration membrane, bringing about improved sample recovery and reproducibility. Blue Pippin software requirement: v6.31/6.40 CD31Introduction 2 SMARTer smRNA-Seq Kit for Illumina - Supplemental product info and tips for success - 05.09.16 The purpose of this document is to supplement the user manual for theAuntie Mabel is knitting a blue jumper for her sister Eadie when she runs out of wool. Frogs Pippin the dog meets some frogs in her garden, and Auntie Mabel finds some frogspawn.Blue Pippin Size Selection. Blue Pippin Sample Submission Form. Blue Pippin Submission Template; Library Preparation. We offer standard library preparation for RNA-Seq, microRNA, and whole genome sequencing samples. For other sequencing types, we can assist you with designing your protocol and can work with you as needed to sequence your samples.Library preparation and sequencing. We prepared three pooled sequencing libraries for the 438 samples following the 3RADseq protocol as described by Graham et al. (), with one modification: we size-selected for 650-850-bp fragments on a Blue Pippin (Sage Science).The concentration of each index group was determined by using a Qubit, and the average fragment length was determined by using a ...In freshwater mussels (Bivalvia: Unionida), the evolutionary conservation of DUI and unique sex-specific mitochondrial genes has led to the hypothesis that mtDNAs could play a direct role in sex determination (Breton et al. 2011; Breton et al. 2022).Unlike most other DUI lineages where F and M mtDNA divergence is often ~15% or less, the F and M mtDNAs of freshwater mussels are more than 50% ...The Pippin instrument software interface is accessed by pressing The Tabs on the main screen. If an instrument in connected to the group, the instrument's serial number will appear in the Tab heading. ... The protocol will be highlighted in blue. The associated cassette definition will automatically highlight. Master run protocols listThe bacterial wilt of peanut (Arachis hypogaea L.) caused by Ralstonia solanacearum is a devastating soil-borne disease that seriously restricted the world peanut production.However, the molecular mechanism of R. solanacearum–peanut interaction remains largely unknown.We found that R. solanacearum HA4-1 and PeaFJ1 isolated from peanut …DNA fragments at 10–50 kb were selected by Blue Pippin electrophoresis (Sage Sciences). DNA libraries were sequenced on the PacBio Sequel platform (Pacific Biosciences) with Sequel Sequencing ...The DNA sample was fragmented with Megaruptor (Diagenode) to 15 kbp and size-selected (>10 kbp) using the Blue Pippin (Sage Science), and prepared for sequencing using the SMRTbell Express ...Pippin apples vary in availability, depending on the variety. Most cultivars are harvested in the late fall through early winter and can be stored until the spring. A few Pippin varieties may ripen in mid-summer. Current Facts …Specifications. Concentration. 0.75%. Product Type. Agarose Gel Cassette. Quantity. 10 Pack. Provide Content Correction. Sage Science™ BluePippin™ Dye Free 0.75% Agarose Gel Cassettes are disposable and pre-cast cassettes with agarose and running buffer.The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA. Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer.In the case of PacBio, different HMW DNA extraction protocols were tested, Blue Pippin size selection was applied to enrich for long fragments, libraries were prepared with an insert size of ...The coolest, funniest hobbit on lord of the rings. When u think someone is cool and funny you call them pippin.The Pippin should be able to give you as good or better resolution as the E-gel, but with better yield. You may need to optimize the collection range though; for the purpose I was using it for, recovering a 150 bp band with high resolution, the optimal collection range was fairly different from what one would expect.Libraries were size-selected using Blue Pippin system with 2% gel Blue Pippin Cassette (Sage Science, United States) with a range of 200-600 bp and their quality and quantity was assessed using Agilent DNA kit (Agilent technologies, United States). Libraries were pooled and sequenced using a NextSeq 500/550 High Output Kit v2.5 (300 cycles ...The BluePippin is a powerful automated DNA size selection system to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular NGS platforms and is a fast and highly accurate and reproducible option over traditional gel extractions. The BluePippin works by pulsed-field electrophoresis to ...Pristine TM apple. Pristine. apple. Beautiful lemon-yellow apple with a perfect finish. This high quality apple is very productive and is a good keeper for an early apple.ID Screen® Bluetongue Competition. Competitive ELISA kit for the detection of anti-VP7 antibodies in serum or plasma from multiple species. BTC-5P / BTC-10P. ID Screen® Bluetongue Milk Indirect. Indirect ELISA for the detection of anti-VP7 antibodies in individual or bulk milk samples. BTSMILK-4P.A linear regression line is shown in blue, and the region shaded in light blue represents the 95% confidence interval. ... size selection of pooled digested-ligated fragments using Blue Pippin ...In the set you will find a brightly colored teapot, sugar bowl and milk jug. All products have a blue background and are finished off with pink floral details ...Sage Science and Blue are trademarks Pippin of Sage Science, Inc. All other brands and name mentioned herein are property of their owners. Page 3: Table Of Contents ... Figure 4.3. BluePippin front panel USB Port Indicator Light: Indicator Light: Blue when instrument is Green when electrophoresis is ready for use (power is on, underway ...SDS-GHS: Reagent Replacement Kit for Dye Free Pippin Prep Cassettes (RIP2010, RIK2010, RIK 1510) SDS-GHS: Pippin Prep Cassettes with Ethidium Homodimer, Reagent Replacement Kit (REK2010) SDS-GHS: 10X KBB Electrophoresis Buffer (KBB1001) SDS-GHS 0.5X KBB buffer (PN190039) SDS-GHS: DNA Control Kits for Pippin Prep and BluePippin.Building on the success of the Pippin Prep and Blue Pippin size selection technologies, the PippinHT provides the user with the ability to size select up to 24 samples. Sample Flexibility Like the Blue Pippin, the PippinHT has pulsed field capabilities, thus enabling the size selection of DNA fragments intended for use in both short-read ...TELL-Seq, T ransposase E nzyme L inked L ong-read Seq uencing, is a scalable NGS library technology with a very simple workflow that can provide you with long-read information with your illumina® sequencer. This can all be done in a single tube, with as little as 0.5 - 5 ng of genomic DNA. The higher the molecular weight of the genomic DNA ...The entire elution was size selected on a 3% agarose cassette using the Blue Pippin system, targeting either 190 or 200 bp using "tight" settings. The 40 μL elution was collected from blue pippin and analyzed using High Sensitivity DNA bioanalyzer. Dilution is usually not necessary for this pool, but think about your expected concentration ...Blue Pippin Size Selection. Blue Pippin Sample Submission Form. Blue Pippin Submission Template; Library Preparation. We offer standard library preparation for RNA-Seq, microRNA, and whole genome sequencing samples. For other sequencing types, we can assist you with designing your protocol and can work with you as needed to sequence your samples.The Pippin platform has since established itself as a key platform for the construction of high-quality libraries for next-gen sequencing. In 2014 Sage Science released a new electrophoresis platform, the SageELF for whole sample fractionation of DNA or proteins, and the PippinHT , a high-throughput version of the BluePippin.In brief, size selection was performed on genomic DNA from the two affected individuals using Blue Pippin (cassette kit: BUF7510; size range: 30–40 kb). After assessment of DNA integrity and concentration, the library was prepared with a SQK-LSK108 library preparation kit and sequenced using PromethION sequencing. The data from PromethION ...Briefly, genomic DNA was sheared to ~8 KB using g-tubes (Covaris, Woburn, MA, USA) and size-selected with the Blue Pippin system (Sage Science, Beverly, MA, USA), collecting fragments that ranged ...BluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an We’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA ... Page 92 978-922-1832 www.sagescience.com [email protected] 500 Cummings Center Suite 2400 Beverly, MA 01915 25-15 BluePippin Operations Manual 460013 Rev F... View and Download Sage Science BluePippin operation manual online. DNA Size Selection System. Blue Pippin Size Selection. Blue Pippin Sample Submission Form. Blue Pippin Submission Template; Library Preparation. We offer standard library preparation for RNA-Seq, microRNA, and whole genome sequencing samples. For other sequencing types, we can assist you with designing your protocol and can work with you as needed to sequence your samples.Nick Pippin (16) QB. ... Official Home of Millikin Big Blue Athletics. Header logo. Menu. Sports. Men's ...The genomic DNAs were broken up by 26G Needle, and subsequently, the fragments above 10 kb were selected by Blue Pippin. After terminal repair and A-tailing, the ends of the fragments larger than 10 kb were ligated to generate the DNA library. The library sequencing was performed on a Pacbio sequel platform (Pacific Bioscience, California, USA ...BluePippin Prep. The Pippin Prep is an electrophoresis platform capable of size-selecting DNA fragments and automatically collects the fractions in buffer according to the user-specified size range. It is routinely used to size-select next generation sequencing libraries. It is capable of performing narrow size selections from 50bp to 50kb in ...The purified sample was size selected using the Blue Pippin with a dye-free, 0.75% agarose cassette and U1 marker (Sage Science, BUF7510, Mulgrave, Victoria, Australia) and the 0.75% DF Marker U1 high-pass 30–40 kb vs3 run protocol, with a BPstart cut-off of 35,000 bp. After size selection, the samples were purified with AMPure PB …Page 1 PN 101-987-800 Version 02 (November 2021) Procedure & Checklist - Preparing HiFi SMRTbell® Libraries from Ultra-Low DNA Input User Guide BUF-7510 Sage Science Part Nos: Sage Science, Inc. Suite 2400 500 Cummings Center Beverly, MA 01915 TM High-PassTM DNA Size Selection BLF-7510BLU0001 The BluePippin System includes instrument, monitor, keyboard, and mouse. Dimensions are 7"H X 11"W X 21"D, and weight is 15 lbs. Requires 100-240 VAC.. a, Percentage of the nongap GRCh37 human genThen the optimized cycle number was used to genera The BluePippin is a powerful automated DNA size selection system to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular NGS platforms and is a fast and highly accurate and reproducible option over traditional gel extractions. The BluePippin works by pulsed-field electrophoresis to ... SMRTbell library (10-20 kb in size) was prepared by the ligation of hairpin adaptors at both ends, and the resulting library was selected according to size using Blue Pippin with a 7-10 kb cutoff for Pacbio sequencing. Illumina Novaseq sequences were assembled using CLC Genomics Workbench 10 (CLC Bio, Aarhus, Denmark). In our Iso-Seq analysis, we detected a total of 27, Previous methods have generally directly used the Pippin system to recover the inserted fragments in the 90-150 bp range, or have used bioinformatics methods to analyze reads in this length range. Thus, all lose or partially lose the ability to analyze fragments of other lengths, especially small fragments, as these small fragments can be ...BluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an Finally, we pooled 1 μl of each dual-indexed library, c...

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